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The Hi Seq 2500 System is a powerful and efficient ultra-high-throughput sequencing system that supports the broadest range of applications and study sizes.
Unrivaled data quality using Illumina's proven SBS chemistry has made the Hi Seq 2500 the instrument of choice for all major genome centers and leading institutions throughout the world.
Automated and high-throughput partitioning by droplet reactions.Unique barcoding of transcripts from each droplet allow transcriptome profiling of thousands of cells simultaneously.In DNA-Seq (WGS and exome) applications, HMW-DNA (50-100kbp) is partitioned, allowing haplotype specific fragments to be uniquely barcoded.Short reads, linked by barcode sharing, are used for phased SNP calling, structural variants identification and long range diploid genome assembly. DNA and RNA extraction is routinely performed at IGATech, providing support to our customers directly from raw material.Relying on automated systems and consolidated protocols we can help you in accelerating the path to analyzed data.We also work with non standard substrates, such as plants and food.Our expertise in the isolation of high molecular weigth DNA for long-molecule sequencing applications will also be a specialized complement for The whole genome sequence as the most comprehensive overview of its composition.Identify most of the variability accounted by SNPs, CNVs, PAVs, insertions, deletions, translocations along with its functional impact on the genetic code an organism.Dedicated technologies are applied for Providing the same type of information that can be yield from a whole genome sequencing, but focusing only on the coding part of a genome.Most of the phenotypically relevant variants reside on the coding regions or their nearby regions.Further enrichment can be carried out on a very limited set of genes: this is useful to reduce costs but also to produce very high coverage on such genes, providing adequate depth to detect somatic variations in tumor samples. Reducing the complexity of genomes has enabled NGS to pursue massive genotyping experiments with very competitive costs.